SrasV1, Main, Exploration, bibRecord, 001C81

Protective effects of long pentraxin PTX3 on lung injury in a severe acute respiratory syndrome model in mice

Identifieur interne : 001C81 ( Main/Exploration ); précédent : 001C80; suivant : 001C82

Protective effects of long pentraxin PTX3 on lung injury in a severe acute respiratory syndrome model in mice

Auteurs : Bing Han ; Xuezhong Ma ; Jianhua Zhang ; Yu Zhang ; Xiaohui Bai ; David M. Hwang ; Shaf Keshavjee ; Gary A. Levy ; Ian Mcgilvray ; Mingyao Liu

Source :

Laboratory Investigation; a Journal of Technical Methods and Pathology [ 0023-6837 ] ; 2012.

RBID : PMC:3955193

Descripteurs français

KwdFr :
- Animaux, Charge virale, Modèles animaux de maladie humaine, Protéine C-réactive (génétique), Protéine C-réactive (physiologie), Protéines de tissu nerveux (génétique), Protéines de tissu nerveux (physiologie), Souris, Souris knockout, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (virologie), Test ELISA.
MESH :
- génétique : Protéine C-réactive, Protéines de tissu nerveux.
- physiologie : Protéine C-réactive, Protéines de tissu nerveux.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Charge virale, Modèles animaux de maladie humaine, Souris, Souris knockout, Syndrome respiratoire aigu sévère, Test ELISA.

English descriptors

KwdEn :
- Animals, C-Reactive Protein (genetics), C-Reactive Protein (physiology), Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Mice, Mice, Knockout, Nerve Tissue Proteins (genetics), Nerve Tissue Proteins (physiology), Severe Acute Respiratory Syndrome (prevention & control), Severe Acute Respiratory Syndrome (virology), Viral Load.
MESH :
- chemical , genetics : C-Reactive Protein, Nerve Tissue Proteins.
- chemical , physiology : C-Reactive Protein, Nerve Tissue Proteins.
- prevention & control : Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Mice, Mice, Knockout, Viral Load.

Abstract

The outbreak of severe acute respiratory syndrome (SARS) in 2003 reinforces the potential of lethal pandemics of respiratory viral infections. The underlying mechanisms of SARS are still largely undefined. Long pentraxin PTX3, a humoral mediator of innate immunity, has been reported to have anti-viral effects. We examined the role of PTX3 in coronavirus murine hepatitis virus strain 1 (MHV-1)-induced acute lung injury, a previously reported animal model for SARS. PTX3-deficient mice (129/SvEv/C57BL6/J) and their wild-type (WT) littermates were intranasally infected MHV-1. These mice were also treated with recombinant PTX3. Effects of PTX3 on viral binding and infectivity were determined in vitro. Cytokine expression, severity of lung injury, leukocyte infiltration and inflammatory responses were examined in vivo. In PTX3 WT mice, MHV-1 induced PTX3 expression in the lung and serum in a time-dependent manner. MHV-1 infection led to acute lung injury with greater severity in PTX3-deficient mice than that in WT mice. PTX3 deficiency enhanced early infiltration of neutrophils and macrophages in the lung. PTX3 bound to MHV-1 and MHV-3 and reduced MHV-1 infectivity in vitro. Administration of recombinant PTX3 significantly accelerated viral clearance in the lung, attenuated MHV-1-induced lung injury, and reduced early neutrophil influx and elevation of inflammatory mediators in the lung. Results from this study indicate a protective role of PTX3 in coronaviral infection-induced acute lung injury.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3955193

DOI: 10.1038/labinvest.2012.92
PubMed: 22732935
PubMed Central: 3955193

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p id="Par1">The outbreak of severe acute respiratory syndrome (SARS) in 2003 reinforces the potential of lethal pandemics of respiratory viral infections. The underlying mechanisms of SARS are still largely undefined. Long pentraxin PTX3, a humoral mediator of innate immunity, has been reported to have anti-viral effects. We examined the role of PTX3 in coronavirus murine hepatitis virus strain 1 (MHV-1)-induced acute lung injury, a previously reported animal model for SARS. PTX3-deficient mice (129/SvEv/C57BL6/J) and their wild-type (WT) littermates were intranasally infected MHV-1. These mice were also treated with recombinant PTX3. Effects of PTX3 on viral binding and infectivity were determined <italic>in vitro</italic>
. Cytokine expression, severity of lung injury, leukocyte infiltration and inflammatory responses were examined <italic>in vivo</italic>
. In PTX3 WT mice, MHV-1 induced PTX3 expression in the lung and serum in a time-dependent manner. MHV-1 infection led to acute lung injury with greater severity in PTX3-deficient mice than that in WT mice. PTX3 deficiency enhanced early infiltration of neutrophils and macrophages in the lung. PTX3 bound to MHV-1 and MHV-3 and reduced MHV-1 infectivity <italic>in vitro</italic>
. Administration of recombinant PTX3 significantly accelerated viral clearance in the lung, attenuated MHV-1-induced lung injury, and reduced early neutrophil influx and elevation of inflammatory mediators in the lung. Results from this study indicate a protective role of PTX3 in coronaviral infection-induced acute lung injury.</p>
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<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Lagace Wiens, Pr" uniqKey="Lagace Wiens P">PR Lagace-Wiens</name>
</author>
<author><name sortKey="Rubinstein, E" uniqKey="Rubinstein E">E Rubinstein</name>
</author>
<author><name sortKey="Gumel, A" uniqKey="Gumel A">A Gumel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lapinsky, Se" uniqKey="Lapinsky S">SE Lapinsky</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name>
</author>
<author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name>
</author>
<author><name sortKey="Li, Ks" uniqKey="Li K">KS Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Albuquerque, N" uniqKey="De Albuquerque N">N De Albuquerque</name>
</author>
<author><name sortKey="Baig, E" uniqKey="Baig E">E Baig</name>
</author>
<author><name sortKey="Ma, X" uniqKey="Ma X">X Ma</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dealbuquerque, N" uniqKey="Dealbuquerque N">N DeAlbuquerque</name>
</author>
<author><name sortKey="Baig, E" uniqKey="Baig E">E Baig</name>
</author>
<author><name sortKey="Xuezhong, M" uniqKey="Xuezhong M">M Xuezhong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name>
</author>
<author><name sortKey="Deming, D" uniqKey="Deming D">D Deming</name>
</author>
<author><name sortKey="Paddock, Cd" uniqKey="Paddock C">CD Paddock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Day, Cw" uniqKey="Day C">CW Day</name>
</author>
<author><name sortKey="Baric, R" uniqKey="Baric R">R Baric</name>
</author>
<author><name sortKey="Cai, Sx" uniqKey="Cai S">SX Cai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Khanolkar, A" uniqKey="Khanolkar A">A Khanolkar</name>
</author>
<author><name sortKey="Hartwig, Sm" uniqKey="Hartwig S">SM Hartwig</name>
</author>
<author><name sortKey="Haag, Ba" uniqKey="Haag B">BA Haag</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hwang, Dm" uniqKey="Hwang D">DM Hwang</name>
</author>
<author><name sortKey="Chamberlain, Dw" uniqKey="Chamberlain D">DW Chamberlain</name>
</author>
<author><name sortKey="Poutanen, Sm" uniqKey="Poutanen S">SM Poutanen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rota, Pa" uniqKey="Rota P">PA Rota</name>
</author>
<author><name sortKey="Oberste, Ms" uniqKey="Oberste M">MS Oberste</name>
</author>
<author><name sortKey="Monroe, Ss" uniqKey="Monroe S">SS Monroe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ma, Xz" uniqKey="Ma X">XZ Ma</name>
</author>
<author><name sortKey="Bartczak, A" uniqKey="Bartczak A">A Bartczak</name>
</author>
<author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bottazzi, B" uniqKey="Bottazzi B">B Bottazzi</name>
</author>
<author><name sortKey="Doni, A" uniqKey="Doni A">A Doni</name>
</author>
<author><name sortKey="Garlanda, C" uniqKey="Garlanda C">C Garlanda</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Garlanda, C" uniqKey="Garlanda C">C Garlanda</name>
</author>
<author><name sortKey="Bottazzi, B" uniqKey="Bottazzi B">B Bottazzi</name>
</author>
<author><name sortKey="Bastone, A" uniqKey="Bastone A">A Bastone</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Deban, L" uniqKey="Deban L">L Deban</name>
</author>
<author><name sortKey="Bottazzi, B" uniqKey="Bottazzi B">B Bottazzi</name>
</author>
<author><name sortKey="Garlanda, C" uniqKey="Garlanda C">C Garlanda</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Muller, B" uniqKey="Muller B">B Muller</name>
</author>
<author><name sortKey="Peri, G" uniqKey="Peri G">G Peri</name>
</author>
<author><name sortKey="Doni, A" uniqKey="Doni A">A Doni</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="He, X" uniqKey="He X">X He</name>
</author>
<author><name sortKey="Han, B" uniqKey="Han B">B Han</name>
</author>
<author><name sortKey="Liu, M" uniqKey="Liu M">M Liu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Garlanda, C" uniqKey="Garlanda C">C Garlanda</name>
</author>
<author><name sortKey="Hirsch, E" uniqKey="Hirsch E">E Hirsch</name>
</author>
<author><name sortKey="Bozza, S" uniqKey="Bozza S">S Bozza</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Soares, Ac" uniqKey="Soares A">AC Soares</name>
</author>
<author><name sortKey="Souza, Dg" uniqKey="Souza D">DG Souza</name>
</author>
<author><name sortKey="Pinho, V" uniqKey="Pinho V">V Pinho</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mairuhu, At" uniqKey="Mairuhu A">AT Mairuhu</name>
</author>
<author><name sortKey="Peri, G" uniqKey="Peri G">G Peri</name>
</author>
<author><name sortKey="Setiati, Te" uniqKey="Setiati T">TE Setiati</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bozza, S" uniqKey="Bozza S">S Bozza</name>
</author>
<author><name sortKey="Bistoni, F" uniqKey="Bistoni F">F Bistoni</name>
</author>
<author><name sortKey="Gaziano, R" uniqKey="Gaziano R">R Gaziano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reading, Pc" uniqKey="Reading P">PC Reading</name>
</author>
<author><name sortKey="Bozza, S" uniqKey="Bozza S">S Bozza</name>
</author>
<author><name sortKey="Gilbertson, B" uniqKey="Gilbertson B">B Gilbertson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Smits, Sl" uniqKey="Smits S">SL Smits</name>
</author>
<author><name sortKey="De Lang, A" uniqKey="De Lang A">A de Lang</name>
</author>
<author><name sortKey="Van Den Brand, Jm" uniqKey="Van Den Brand J">JM van den Brand</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Varani, S" uniqKey="Varani S">S Varani</name>
</author>
<author><name sortKey="Elvin, Ja" uniqKey="Elvin J">JA Elvin</name>
</author>
<author><name sortKey="Yan, C" uniqKey="Yan C">C Yan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rivieccio, V" uniqKey="Rivieccio V">V Rivieccio</name>
</author>
<author><name sortKey="Esposito, A" uniqKey="Esposito A">A Esposito</name>
</author>
<author><name sortKey="Bellofiore, P" uniqKey="Bellofiore P">P Bellofiore</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Levy, Ga" uniqKey="Levy G">GA Levy</name>
</author>
<author><name sortKey="Leibowitz, Jl" uniqKey="Leibowitz J">JL Leibowitz</name>
</author>
<author><name sortKey="Edgington, Ts" uniqKey="Edgington T">TS Edgington</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mura, M" uniqKey="Mura M">M Mura</name>
</author>
<author><name sortKey="Binnie, M" uniqKey="Binnie M">M Binnie</name>
</author>
<author><name sortKey="Han, B" uniqKey="Han B">B Han</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Han, B" uniqKey="Han B">B Han</name>
</author>
<author><name sortKey="Mura, M" uniqKey="Mura M">M Mura</name>
</author>
<author><name sortKey="Andrade, Cf" uniqKey="Andrade C">CF Andrade</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="He, X" uniqKey="He X">X He</name>
</author>
<author><name sortKey="Han, B" uniqKey="Han B">B Han</name>
</author>
<author><name sortKey="Bai, X" uniqKey="Bai X">X Bai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="He, X" uniqKey="He X">X He</name>
</author>
<author><name sortKey="Han, B" uniqKey="Han B">B Han</name>
</author>
<author><name sortKey="Mura, M" uniqKey="Mura M">M Mura</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, C" uniqKey="Li C">C Li</name>
</author>
<author><name sortKey="Fung, Ls" uniqKey="Fung L">LS Fung</name>
</author>
<author><name sortKey="Chung, S" uniqKey="Chung S">S Chung</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Jh" uniqKey="Chen J">JH Chen</name>
</author>
<author><name sortKey="Chang, Yw" uniqKey="Chang Y">YW Chang</name>
</author>
<author><name sortKey="Yao, Cw" uniqKey="Yao C">CW Yao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nathan, C" uniqKey="Nathan C">C Nathan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Okutani, D" uniqKey="Okutani D">D Okutani</name>
</author>
<author><name sortKey="Han, B" uniqKey="Han B">B Han</name>
</author>
<author><name sortKey="Mura, M" uniqKey="Mura M">M Mura</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mauri, T" uniqKey="Mauri T">T Mauri</name>
</author>
<author><name sortKey="Bellani, G" uniqKey="Bellani G">G Bellani</name>
</author>
<author><name sortKey="Patroniti, N" uniqKey="Patroniti N">N Patroniti</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mauri, T" uniqKey="Mauri T">T Mauri</name>
</author>
<author><name sortKey="Coppadoro, A" uniqKey="Coppadoro A">A Coppadoro</name>
</author>
<author><name sortKey="Bellani, G" uniqKey="Bellani G">G Bellani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Han, B" uniqKey="Han B">B Han</name>
</author>
<author><name sortKey="Haitsma, Jj" uniqKey="Haitsma J">JJ Haitsma</name>
</author>
<author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Deban, L" uniqKey="Deban L">L Deban</name>
</author>
<author><name sortKey="Russo, Rc" uniqKey="Russo R">RC Russo</name>
</author>
<author><name sortKey="Sironi, M" uniqKey="Sironi M">M Sironi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Leibowitz, Jl" uniqKey="Leibowitz J">JL Leibowitz</name>
</author>
<author><name sortKey="Srinivasa, R" uniqKey="Srinivasa R">R Srinivasa</name>
</author>
<author><name sortKey="Williamson, St" uniqKey="Williamson S">ST Williamson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fujimoto, I" uniqKey="Fujimoto I">I Fujimoto</name>
</author>
<author><name sortKey="Pan, J" uniqKey="Pan J">J Pan</name>
</author>
<author><name sortKey="Takizawa, T" uniqKey="Takizawa T">T Takizawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pribul, Pk" uniqKey="Pribul P">PK Pribul</name>
</author>
<author><name sortKey="Harker, J" uniqKey="Harker J">J Harker</name>
</author>
<author><name sortKey="Wang, B" uniqKey="Wang B">B Wang</name>
</author>
</analytic>
</biblStruct>
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<name sortKey="Han, Bing" sort="Han, Bing" uniqKey="Han B" first="Bing" last="Han">Bing Han</name>
<name sortKey="Hwang, David M" sort="Hwang, David M" uniqKey="Hwang D" first="David M" last="Hwang">David M. Hwang</name>
<name sortKey="Keshavjee, Shaf" sort="Keshavjee, Shaf" uniqKey="Keshavjee S" first="Shaf" last="Keshavjee">Shaf Keshavjee</name>
<name sortKey="Levy, Gary A" sort="Levy, Gary A" uniqKey="Levy G" first="Gary A" last="Levy">Gary A. Levy</name>
<name sortKey="Liu, Mingyao" sort="Liu, Mingyao" uniqKey="Liu M" first="Mingyao" last="Liu">Mingyao Liu</name>
<name sortKey="Ma, Xuezhong" sort="Ma, Xuezhong" uniqKey="Ma X" first="Xuezhong" last="Ma">Xuezhong Ma</name>
<name sortKey="Mcgilvray, Ian" sort="Mcgilvray, Ian" uniqKey="Mcgilvray I" first="Ian" last="Mcgilvray">Ian Mcgilvray</name>
<name sortKey="Zhang, Jianhua" sort="Zhang, Jianhua" uniqKey="Zhang J" first="Jianhua" last="Zhang">Jianhua Zhang</name>
<name sortKey="Zhang, Yu" sort="Zhang, Yu" uniqKey="Zhang Y" first="Yu" last="Zhang">Yu Zhang</name>
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Protective effects of long pentraxin PTX3 on lung injury in a severe acute respiratory syndrome model in mice

Protective effects of long pentraxin PTX3 on lung injury in a severe acute respiratory syndrome model in mice

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